poplar WT52-30

GMTW-112 , updated 8 Sep 2010
bio-ethanol production

Tree species

Populus alba x P. tremula
Poplar hybrid

Genetic modification

Agrobacterium tumefaciens-mediated transformation

Notifier & developers

Trial locations


2009 - 2014
The site is in total 6500 m2 of which a maximum of 2400 m2 will be planted with transgenic poplars.
University of Ghent

Detailed description

The genetically modified poplars exhibit modified lignin (a major constituent of wood) due to the decreased activity of an enzyme of the lignin biosynthetic pathway. The altered enzyme is CCR (Cinnamoyl coenzymeA reductase). The down-regulation has been obtained by co-suppression [...] The enzyme residual activity varies between 10 to 40 % and is not necessarily uniform within the plant. Consequently, the quality or/and quantity of lignin is modified. These modifications and the consequences on some wood properties have been described in several publications (Baucher et al., 1996, van Doorsselaere et al., 1995 ; Meyermans et al., 2000 ; Lapierre et al., 1999 ; Pilate et al., 2002 ; Lapierre et al., 2004).
In addition, all transgenic lines have also integrated a selection gene (hpt) that confers an antibiotic resistance. This antibiotic resistance has been used during in vitro culture steps to screen for genetically modified cells. (JRC 2007)