6786-01-0145: heavy metal tolerant poplar

GMTW-134 , updated 20 Nov 2010
phytoremediation of soils contaminated with heavy metals

Tree species

Populus alba x P. tremula
Poplar hybrid
INRA Klon 717 1-B4, female
Populus deltoides
Eastern Cottonwood
INRA Klon 717 1-B4, female

Genetic modification

Agrobacterium tumefaciens-mediated transformation

Notifier & developers

Trial locations


2003 - 2005
2 x 2500 m2
Großörner (Mansfeld)

Detailed description

The EU register as well as the German location register list a trial with heavy metal tolerant poplars at the same locations and at the same time. However, according to the JRC registration and the final report the GM poplar is P. deltoides, while the BVL registration lists it as poplar hybrid P. tremula x P. alba.

trait: nptII: Kanamycin- resistance; gshI from E.coli: over-expression of Gamma-Glutamylcystein Synthetase, two- to fourfold higher glutathione concentration [...]
right border from Agrobacterium tumefaciens (161 bp)
Nopalin Synthase Promotor from Agrobacterium tumefaciens (306 bp)
npt-II Gen from Tn5 from E. coli (983 bp) - Kanamycin resistance
Nopalin Synthase Terminator from Agrobacterium tumefaciens (255 bp)
Sequenz from Gen III (441 bp) no relevant function
lacI Gen (141 bp) no relevant function
lacZ Gen + Polylinker (116 bp) no relevant function
35S Terminator from Cauliflower-Mosaic-Virus (725 bp)
gshI Gen from E.coli (1585 bp, Anlage D) - -glutamylcysteine synthetase
35S-Promotor with P70 double-Enhancer from Cauliflower-Mosaic-Virus (750 bp)
lacZ Gen + Polylinker (113 bp)
M13ori (300 bp) no relevant function
lacI Gen (130 bp) no relevant function
left Border from Agrobacterium tumefaciens (147 bp)
(JRC 2002)