fungi resistant apple

GMTW-153 , updated 8 Sep 2010

Tree species

Genetic modification

Agrobacterium tumefaciens-mediated transformation

Notifier & developers

Plant Research International (PRI) Netherlands

Trial locations

Locationsort icon Country
Belgium Belgium
Netherlands Netherlands
Wageningen Netherlands

Europe

unknown
unknown
unknown
Belgium
2003 - 2007
Maximum of 1 site per year. Maximum area of the site 3850 m2
unknown
Wageningen
2004 - 2008
Maximum of 1 site per year. Maximum area of the site 3850 m2
unknown
Wageningen
unknown
unknown
unknown
Netherlands

Detailed description

In some of the trials this tree is specifically described as "scab resistance", in others it is broadly described as "fungus resistant" or even "fungus and bacterial disease resistant."

The genetically modified apple trees are inoculated onto non-modified rootstocks. The GM apples carry a kanamycin resistance gene nptII, a modified beta-glucuronidase gene (GUS-intron) and a hth gene encoding a type 1 alpha-hordothionine precursor conferring resistance to fungi. [...]
The binary vector pMOG402.hth.gusintron contains between the borders the following elements:
-P35S: promoter derived from the CaMV 35S gene for transcription start, consitutive expression and enhanced expression
-T35S: terminator derived from the CaMV 35S gene
-Pnos: promoter derived from the nopaline synthase gene from the Ti plasmid of Agrobacterium tumefaciens
-Tnos: terminator derived from the nopaline synthase gene from the Ti plasmid of Agrobacterium tumefaciens
-nptII: kanamycin resistance gene from Tn5, can be derived from Escherichia coli
-gus(int): B-glucuronidase gene, besides original version also a version with an intron inserted which originates from plants
-hth: alpha-hordothionine gene derived from barley, conferring resistance to fungi. (JRC 2002)