early flowering carrizo citrange with visual marker and ARMG nptII

GMTW-166 , updated 16 Jul 2010

Links & documents

Tree species

Citrus sinensis x Poncirus trifoliata
Carrizo citrange
Citrange

Genetic modification

Agrobacterium tumefaciens-mediated transformation
tissue culture

Notifier & developers

Trial locations

Europe

2008 - 2018
500 m2 in an area of 7500 m2 with four different field trials
unknown
Villareal

Detailed description

"Ectopic overexpression of floral meristem identity genes APETALA 1 (AP1), SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and FLOWERING LOCUS T (FT) from Arabidopsis thaliana in Carrizo citrange to induce early flowering in the transgenic plants and their progeny, thus generating rapid cycling trees. We want know to study whether over-expression of those transgenes modify growth and development of trnasgenic trees growing in the field.
All transformed plants are also carrying uidA and nptII transgenes conferring β-glucuronidase activity and resistance to kanamycin, respectively." (JRC 2008)

"Cassette NOSpro:: nptII:: NOSter: 1758 nucleotides. The nptII gene comes from Tn5 transposon of E. coli. NOS regulatory regions (promoter and terminator) come from the nopaline synthase (NOS) gene from Agrobacterium tumefaciens. Transgene expression confers resistance to kanamycin to the transgenic cells and tissues. This cassette is present in the T-DNA from pROKII, which was used as the binary vector for A. tumefaciens mediated transformation of orange and citrange explants.
Cassette 35Spro::uidA::35SpA: 2800 nucleotides. The uidA gene comes from E. coli. 35S regulatory regions (promoter and terminator) come from the 35S gene from Cauliflower mosaic virus (CaMV). Transgene expression confers β-glucuronidase activity to the transgenic cells and tissues. This cassette is present in the T-DNA from pROKII, which was used as the binary vector for A. tumefaciens mediated transformation of orange and citrange explants.
Cassette 35Spro::AP1:: NOSter: 870, 1054 y 275 nucleotides, respectively.
Cassette 35Spro::FT:: NOSter: 870, 864 y 275 nucleotides, respectively.
Módulo 35Spro::SOC1:: NOSter: 870, 1320 y 275 nucleotides, respectively.
Promoter and terminator regions come from the NOS gene from Agrobacterium tumefaciens, respectively, and they confer high and constitutive expression of the transgene. The AP1, FT and SOC1 transgenes come from Arabidopsis thaliana. Their overexpression induce early flowering in transgenic citrus plants." (JRC 2008)