INRA 353-38: Cry3a Bt producing P. deltoides with ARMG hpt

GMTW-1982 , updated 7 Sep 2010
INRA 353-38

Links & documents

Tree species

Populus deltoides
Eastern Cottonwood

Genetic modification

Agrobacterium tumefaciens-mediated transformation

Notifier & developers

Trial locations

Europe

2008 - 2012
400 m2
unknown
Umeå

Detailed description

"INRA # 353-38 is modified to express the synthetic cry3Aa gene derivate from Bacillus thuringensis ssp. Tenebrionis cry3Aa gene. As a result the plants are resistant to insects especially Coleoptera. The toxin affects both growth and survival of the herbivorous beetles.The marker gene HPT from e-coli gives resistance to hygromycin." (JRC 2008)
"INRA # 353-38 : a synthetic cry3Aa gene derivat from Bacillus thuringensis ssp. Tenebrionis cry3Aa gene was inserted. The intended function was to produce a toxin for insects (most specific to coleoptera). The origin of the terminator mas 3 is Agrobacterium tumefaciens, the HPT marker gene e-coli, the promotor CaMV Cauliflower mosaic virus; and the terminatorgene tml3 Agrobacterium tumefaciens. (see Fig 5) Fig. 5. Structure of binary vector pCGN4019. LB, left T-DNA border; RB, right T-DNA border; 35S, CaMV 35Spromoter; hygromycin B phosphotransferase (HPT) gene; tml, tml 3' region; Mac, hybrid 35S/mannopine synthetase promoter; cryllIA, rebuilt crylIIA gene; mas 3 ', mannopine synthetase 3' region.INRA 717-1-B4: is modified by that COMT cDNA were split into two fragments in a sense and antisense orientation and transferred with Agrobacterium tumefaciens. The intended function is to change the lignin concentration/structure and improve pulping performance. The origin of the terminatorgene 3’ocs is Agrobacterium tumefaciens, the nptII marker gene e-coli, the promotor CaMV Cauliflower mosaic virus; and PNOS-Agrobakterium (see fig below). We will only use one line (line ASCOMT2B) based on transformants from the plasmid pASB (antisense orientation) marked with * in the figure below." (JRC 2008)