INRA 717-1-B4 P. deltoides with lower lignin and ARMG nptII

GMTW-1984 , updated 18 Aug 2011

Links & documents

Tree species

Populus deltoides
Eastern Cottonwood

Genetic modification

Agrobacterium tumefaciens-mediated transformation

Notifier & developers

Trial locations

Europe

2008 - 2012
400 m2
unknown
Umeå

Detailed description

"INRA717-1-B4 has a downregulation of COMT and CAD. As a result the trees contain less lignin (COMT) or has changes in lignin structure (CAD) which improves pulping performance. The marker gene NPTII (neomycin fosfortransferas II gene) from e-coli gives resistance to neomycin." (JRC 2008)
"INRA 717-1-B4: is modified by that COMT cDNA were split into two fragments in a sense and antisense orientation and transferred with Agrobacterium tumefaciens. The intended function is to change the lignin concentration/structure and improve pulping performance. The origin of the terminatorgene 3’ocs is Agrobacterium tumefaciens, the nptII marker gene e-coli, the promotor CaMV Cauliflower mosaic virus; and PNOS-Agrobakterium (see fig below). We will only use one line (line ASCOMT2B) based on transformants from the plasmid pASB (antisense orientation) marked with * in the figure below." (JRC 2008)