non-flowering silver birch

GMTW-203 , updated 13 Sep 2010

Tree species

Betula pendula
Silver birch
Hängebirke
Weißbirke
B. alba
B. verrucosa

Genetic modification

Agrobacterium tumefaciens-mediated transformation

Notifier & developers

Trial locations

Europe

2005 - 2008
unknown
unknown
Joensuu
2009 - 2012
1000 m2
unknown
Joensuu

Detailed description

According to the JRC notification reports
"The flowering is prevented by introducing a barnase gene regulated by an inflorescence specific birch promoter (BpFULL1)."
The Inserted transgenic construct consists out of

  • " RB (right border), insertion boundary (Agrobacterium tumefaciens).
  • the promoter sequence (Nos-pro) from the T-DNA nopaline synthase (nos) gene (Agrobacterium tumefaciens).
  • the bacterial nptII gene (Escherichia coli). It codes for the enzyme neomycin phosphotransferase II (NPTII), which gives resistance to the antibiotic kanamycin.
  • the 3’ untranslated region of the nopaline synthase gene (nos-term) from Agrobacterium tumefaciens, containing plant polyadenylation signals
  • the promoter of BpFULL1 gene from birch (Betula pendula), regulates the expression of the barnase gene.
  • the coding region of the barnase gene (without signal sequence) from Bacillus amyloliquefaciens. The expression of the gene in inflorescence-forming cells leads to the death of the cells through the action of the RNase-enzyme coded by the gene.

  • barstar gene from Bacillus amyloliquefaciens. Not expressed in plant cells.
  • the 3’ untranslated region of the nopaline synthase gene (nos-term) from Agrobacterium tumefaciens, containing plant polyadenylation signals
  • LB (left border), insertion boundary (Agrobacterium tumefaciens)." (JRC 2005, 2008)