nptII

Alternative names: 
neomycin phosphotransferase II
Gene: 
Donor: 
Promotor: 
Traits: 
ARMG nptII
Used in: 
Apple rootstock M26
Used in: 
Apple rootstock M9

Neomycin phosphotransferase II (nptII) conveys resistance against a number of antibiotics including kanamycin and neomycin.
The WHO lists Kanamycin as a reserve antibiotic against multiple-resistant tuberculosisi in its WHO Essential Medicines Library
nptII is the same antibiotic resistance marker gene (ARMG) that is also used in the GM potato Amflora. It's classification as an ARMG that can be used in GM crops by the European Food Safety Authority (EFSA) has been heavily criticised including by the European Medicines Agency (EMEA) that stated the concerned antibiotics are used more often then EFSA assumed.

Cassette NOSpro:: nptII:: NOSter: 1758 nucleotides. The nptII gene comes from Tn5 transposon of E. coli. NOS regulatory regions (promoter and terminator) come from the nopaline synthase (NOS) gene from Agrobacterium tumefaciens. Transgene expression confers resistance to kanamycin to the transgenic cells and tissues. This cassette is present in the T-DNA from pBI121, which was used as the binary vector for A. tumefaciens mediated transformation of orange explants (GenBank accesión of pBI121: nº AF485783). (JRC 2008)

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